When migrating to Office 365 the internal outlook users are not able to use autodiscover.
This is because the internal exchange server also uses autodiscover in IIS.
Use this description to remove the internal AutodiscoverVirtualDirectory
Be sure that this is the proper migration plan for you’re organization!
1. Open an elevated command prompt and back-up the IIS configuration (Just in case !):
cd %windir%system32inetsrvappcmd.exe add backup “Before Removing Autodiscover”
2. Open an elevated Exchange Management Shell and retrieve the current autodiscover virtual directory:
Get-AutodiscoverVirtualDirectory | fl Name, Server, InternalUrl, Identity
Copy the Identity value to the clipboard.
3. In the Exchange Management Shell, remove the autodiscover virtual directory:
Remove-AutodiscoverVirtualDirectory -Identity “ALPHAAutodiscover (Default Web Site)”
When you’re identity contains a space, use the quotation marks ”
You will have to confirm by typing a “Y”.
4. Check that the autodiscover virtual directory is gone:
Get-AutodiscoverVirtualDirectory | fl Name, Server, InternalUrl, Identity
This should now return nothing.
5. Now, with Outlook running on a desktop, hold the Ctrl button, right-click on the Outlook icon in the system tray, and select Test E-mail AutoConfiguration. Enter your email address and password and click the Test button. The results should come from the Office 365 server.